LOCUS       sequence-119821-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BATF3
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(449..473)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      697..743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(821..837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(834..856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    845..861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(869..875,876..893,894..899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    914..935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt
       61 ggcaccatgt cgcaagggct cccggccgcc ggcagcgtcc tgcagaggag cgtcgcggcg
      121 cccgggaacc agccgcagcc gcagccgcag cagcagagcc ctgaggatga tgacaggaag
      181 gtccgaagga gagaaaaaaa ccgagttgct gctcagagaa gtcggaagaa gcagacccag
      241 aaggctgaca agctccatga ggaatatgag agcctggagc aagaaaacac catgctgcgg
      301 agagagatcg ggaagctgac agaggagctg aagcacctga cagaggcact gaaggagcac
      361 gagaagatgt gcccgctgct gctctgccct atgaactttg tgccagtgcc tccccggccg
      421 gaccctgtgg ccggctgctt gccccgattg ccaactttct tgtacaaagt ggtgtaagcg
      481 gccgccctcc tcctcctttc ttgttccttt cacgtcgcct tctcggttgt agctggcaga
      541 cgacgagtct tacttttacg tgtacttctc tatagatgat gtatgatctc tctgcatgcg
      601 tgttcgtgca tgtgtccgtg tgttgggtac gcgtggtacc ctgcaggaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag taattactag cataacccct tggggcctct
      721 aaacgggtct tgagggggtt ttttgctgaa aggaggacag ctgatgattg tcatgcttgc
      781 catctgtttt cttgcaaggt cagaggaatt cgtaatcatg gtcatagctg tttcctgtgt
      841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      961 tccagtcggg aaacctgtcg tgccagctgc att
//
