LOCUS       sequence-119823-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2E1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgagctata caagctggag gtgctcttcc gggtccgaca agtttgtaca aaaaagcaga
       61 agggccgtca aggcccacca tggcagaccc agatgtcctc actgaagttc cagcagcatt
      121 gaagcggtta gccaagtatg tgatccgggg attttatggc attgagcatg ccttggcctt
      181 ggacatcttg atcaggaact cctgtgtgaa agaggaggat atgctggagc tgctcaagtt
      241 tgatcggaag caacttcgat cagttttgaa taatttaaag ggagacaagt ttatcaaatg
      301 cagaatgagg gtagagactg ctgcagacgg gaaaaccact cgccataact actacttcat
      361 caattatcgt actcttgtta atgtggtaaa atataaactg gaccacatga gaagaagaat
      421 tgagaccgat gagagagatt cgaccaaccg ggcttccttc aaatgtcctg tctgtagtag
      481 tactttcaca gacttagaag ctaatcagct ctttgatcct atgacaggaa ctttccgctg
      541 tactttttgc catacagagg tagaagagga tgaatcagca atgcccaaaa aagatgcacg
      601 cacacttttg gcaaggttta atgaacaaat tgagcccatt tatgcattgc ttcgggagac
      661 agaggatgtg aacttggcct atgaaatact tgagccagaa cccacagaaa tcccagccct
      721 gaaacagagc aaggaccatg cagcaactac tgctggagct gctagcctag caggtgggca
      781 ccaccgggaa gcatgggcca ccaaaggtcc ttcctatgaa gacttataca ctcagaatgt
      841 tgtcattaac atggatgacc aagaagatct tcatcgagcc tcactggaag ggaaatctgc
//
