LOCUS       sequence-119826-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2E1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtaccacg cgtacccaac acacggacac atgcacgaac acgccatgca gagagatcat
       61 acatcatcta tagagaagta cacgtaaaag taagactcgt cgtctgccag ctacaaccga
      121 gaaggcgacg tgaaaggaac aagaaaggag gaggagggcg gccgcttaca ccactttgta
      181 caagaaagct gggcccatga ggcctgactc aaagaggtcc tcaaacatgc gttgtcccat
      241 tgctatatat gcttcctttt cttctggtgt catctgggcc actagctctg gccgttggct
      301 cacttcactg taggagaacg gacggccagc caccatgaca atggggtcat ctgctacttc
      361 ttcaaactcg tcatcttcct cttcatcctc ttctcgttta tgcacagcca cagctgccgg
      421 acggggtgga gaatcatcat ctgactcact ggtctcgctt tctgagtcac tgccattggc
      481 agcggtcact ggagcagctg cccccactga accagccatg gcagaggaag tctttttctc
      541 gtgaatgagc agtgctcgca tgacctcttc gttgtcatca ggcccagcat ggccttcctc
      601 acgctcctga aatgcgtcca tatctatgcc cccttctttc atatcttcag aaccatatgc
      661 cccttggaca gtgctttctc tcaaccaaat aggcctctct ttggcagatt tcccttccag
      721 tgaggctcga tgaagatctt cttggtcatc catgttaatg acaacattct gagtgtataa
      781 gtcttcatag gaaggacctt tggtggccca tgcttcccgg tggtgcccac ctgctaggct
      841 agcagctcca gcagtagttg ctgcatggtc cttgctctgt ttcagggctg ggatttctgt
//
