LOCUS       sequence-119832-        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..731
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             complement(1539..1550)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcgagctg attaaggaga
       61 acatgcccat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
      121 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtcgtcgagg
      181 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
      241 ccttcatcaa gcaccctccg ggcatccccg acttctttaa gcagtccttc cctgagggct
      301 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
      361 ccagcctcca ggacggctgc ctcatctaca acgtcaaggt tagaggggtg aacttcccag
      421 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagacgatgt
      481 accccgctga cggcggcctg gaaggcgcat gtgacatggc cctgaagctc gtgggcgggg
      541 gccacctgat ctgcaacctt gagaccacat acagatccaa gaaacccgct acgaacctca
      601 agatgcccgg cgtctacaac gtggaccaca gactggaaag aatcaaggag gccgacgatg
      661 agacctacgt cgagcagcac gaggtggctg tggccagata ctctactggt ggcgctggtg
      721 atggaggtaa aggtggagga ggttccggac tcagatctcg agccatgaag ccaggattca
      781 gtccccgtgg gggtggcttt ggcggccgag ggggctttgg tgaccgtggt ggtcgtggag
      841 gccgaggggg ctttggcggg ggccgaggcc aaggcggagg ctttagaggt cgtggacgag
      901 gaggaggtgg aggcggcggc ggcggtggag gaggaggaag aggtggtgga ggcttccatt
      961 ttggtggcaa ccggggtcgt ggtcggggag gaaaaagagg aaaccagtcg gggaagaatg
     1021 tgatggtgga gccgcatcgg catgagggtg tcttcatttg tcggggaaag gaagatgcac
     1081 tggtcaccaa gaacctggtc cctggggaat cagtttatgg agagaagaga gtctcgattt
     1141 cggaaggaga tgacaaaatt gagtaccgag cctggaaccc cttccgctcc aagcttgcag
     1201 cagcaatcct gggtggtgtg gaccagatcc acatcaaacc gggggctaag gttctctacc
     1261 tcggggctgc ctcgggcacc acggtctccc atgtctctga catcgttggt ccggatggtc
     1321 tagtctatgc agtcgagttc tcccaccgct ctggccgtga cctcattaac ttggccaaga
     1381 agaggaccaa catcattcct gtgatcgagg atgctcgaca cccacacaaa taccgcatgc
     1441 tcatcgcaat ggtggatgtg atctttgctg atgtggccca gccagaccag acccggattg
     1501 tggccctgaa tgcccacacc ttcctgcgta atggaggaca ctttgtgatt tccattaagg
     1561 ccaactgcat tgactccaca gcctcagccg aggccgtgtt tgcctccgaa gtgaaaaaga
     1621 tgcaacagga gaacatgaag ccgcaggagc agttgaccct tgagccatat gaaagagacc
     1681 atgccgtggt cgtgggagtg tacaggccac cccccaaggt gaagaactga ggatccaccg
     1741 gatctagata actgatcata atcagccata ccaca
//
