LOCUS       sequence-119844-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL2
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     complement(593..652)
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     protein_bind    complement(652..676)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(677..700)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(743..764)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaagtcagt gttgagatga tgctttgaca aaaggtaatc catctgttca
      241 gaaattctac aatggttgct gtctcatcag catattcaca catgaatgtt gtttcagatc
      301 cctttagttc cagaactatt acgttgatat tgctgattaa gtccctgggt cttaagtgaa
      361 agtttttgct ttgagctaaa tttagcactt cctccagagg tttgagttct tcttctagac
      421 actgaagatg tttcagttct gtggccttct tgggcatgta aaacttaaat gtgagcatcc
      481 tggtgagttt gggattcttg taattattaa ttccattcaa aatcatctgt aaatccagca
      541 gtaaatgctc cagttgtagc tgtgttttct ttgtagaact tgaagtaggt gcactgtttg
      601 tgacaagtgc aagacttagt gcaatgcaag acaggagttg catcctgtac atgccaactt
      661 ttttgtacaa acttgtcgga ccctggaaga gcacctccag cttgtatagc tcgtccatgc
      721 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      781 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      841 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      901 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      961 ta
//
