LOCUS       sequence-119848-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BTF3L4
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(666..690)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(691..714)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(757..778)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtccacgcg tacccaacac acggacacat gcacgaacac gcatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg tagttagctt cattctttga tgcctcatca aaattttcta caagatctgg
      241 aacatcatca tcttcctcat caatgtcttc tggttttggt gctttactgt ccaagacttg
      301 ccgtgggaac tgttcagcta acttcctaag gcttgttaaa ctgtcagcac caagctgact
      361 taatattcca ggaagcattt ctgtgattgg tttggcttct gcatgaccag taattgcaaa
      421 ggtattagca gaaagggaag cttggacttt gggattgttg aaatgaataa ctgtcccatc
      481 atctttaatc atgttcacct cttcaatacc agctatatta ttcacagcca gtttttttag
      541 agaactctga agctttttgt catcagctgt ggctgttcta tgtaccacct tcttctttct
      601 gcgagctgta cccttgcccc ctatccggac ctgagcctga agtttggcta acttttcttg
      661 attcatgcca acttttttgt acaaacttgt cggaccctgg aagagcacct ccagcttgta
      721 tagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      781 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      841 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      901 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      961 cttgtcggcc at
//
