LOCUS       sequence-119857-       10054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IMCE attB donor copy control fosmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10054)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH145
FEATURES             Location/Qualifiers
     source          1..10054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     273..295
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     287..304
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     288..304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     311..330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        311..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    359..392
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1462..1495
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     oriT            1670..1779
                     /note="incP origin of transfer"
     CDS             1812..2183
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     complement(2358..2374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2358..2374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2371..2393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2382..2398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2406..2412,2413..2430,2431..2436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2451..2472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2692..3351)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3266..3285
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3352..3454)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4312..4926
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4403..4934
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5002..5221
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             5312..6067
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    6070..6320
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6646..7821
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7821..8792
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    8865..9338
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    9598..9996
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(10014..10047)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccgt
      361 gccagggcgt gcccttgggc tccccgggcg cgcacgtgcg tgttgcccca gcaatcagcg
      421 cgaccttgcc cctccaacgt catctcgttc tccgctcatg agctcagcca atcgactggc
      481 gagcggcatc gcattcttcg catcccgccc tctggcggat gcaggaagat caacggatct
      541 cggcccagtt gacccagggc tgtcgccaca atgtcgcggg agcggatcaa ccgagcaaag
      601 gcatgaccga ctggaccttc cttctgaagg ctcttctcct tgagccacct gtccgccaag
      661 gcaaagcgct cacagcagtg gtcattctcg agataatcga cgcgtaccaa cttgccatcc
      721 tgaagaatgg tgcagtgtct cggcacccca tagggaacct ttgccatcaa ctcggcaaga
      781 tgcagcgtcg tgttggcatc gtgtcccacg ccgaggagaa gtacctgccc atcgagttca
      841 tggacacggg cgaccgggct tgcaggcgag tgaggtggca ggggcaatgg atcagagatg
      901 atctgctctg cctgtggccc cgctgccgca aaggcaaatg gatgggcgct gcgctttaca
      961 tttggcaggc gccagaatgt gtcagagaca actccaaggt ccggtgtaac gggcgacgtg
     1021 gcaggatcga acggctcgtc gtccagacct gaccacgagg gcatgacgag cgtccctccc
     1081 ggacccagcg cagcacgcag ggcctcgatc agtccaagtg gcccatcttc gaggggccgg
     1141 acgctacgga aggagctgtg gaccagcagc acaccgccgg gggtaacccc aaggttgaga
     1201 agctgaccga tgagctcggc ttttcgccat tcgtattgca cgacattgca ctccaccgct
     1261 gatgacatca gtcgatcata gcacgatcaa cggcactgtt gcaaatagtc ggtggtgata
     1321 aacttatcat ccccttttgc tgatggagct gcacatgaac ccattcaaag gccggcattt
     1381 tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca tcagttaccg
     1441 tgagccggag gatccacgtg acgcgcccgg ggagcccaag ggcacgccct ggcacggatc
     1501 ctctagagtc gacctgcagg catgcaagct tgcgcttttc cgctgcataa ccctgcttcg
     1561 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     1621 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     1681 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     1741 cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg
     1801 agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg
     1861 tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga
     1921 gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact
     1981 atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg ggcggcctgc
     2041 tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg
     2101 ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg atgggcgtgg
     2161 tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg ggtgcgcgtg
     2221 attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtgaag
     2281 tacatcaccg acgagcaagg caagaccgaa agcttgagta ttctatagtc tcacctaaat
     2341 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2521 cagctgcatt aatgaatcgg ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa
     2581 ttctcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat tatcacttat
     2641 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     2701 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     2761 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     2821 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     2881 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     2941 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     3001 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     3061 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     3121 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     3181 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     3241 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     3301 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     3361 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     3421 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     3481 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     3541 cgtcacaggt atttattcgc gataagctca tggagcggcg taaccgtcgc acaggaagga
     3601 cagagaaagc gcggatctgg gaagtgacgg acagaacggt caggacctgg attggggagg
     3661 cggttgccgc cgctgctgct gacggtgtga cgttctctgt tccggtcaca ccacatacgt
     3721 tccgccattc ctatgcgatg cacatgctgt atgccggtat accgctgaaa gttctgcaaa
     3781 gcctgatggg acataagtcc atcagttcaa cggaagtcta cacgaaggtt tttgcgctgg
     3841 atgtggctgc ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg gttgcgatgc
     3901 tgaaacaatt atcctgagaa taaatgcctt ggcctttata tggaaatgtg gaactgagtg
     3961 gatatgctgt ttttgtctgt taaacagaga agctggctgt tatccactga gaagcgaacg
     4021 aaacagtcgg gaaaatctcc cattatcgta gagatccgca ttattaatct caggagcctg
     4081 tgtagcgttt ataggaagta gtgttctgtc atgatgcctg caagcggtaa cgaaaacgat
     4141 ttgaatatgc cttcaggaac aatagaaatc ttcgtgcggt gttacgttga agtggagcgg
     4201 attatgtcag caatggacag aacaacctaa tgaacacaga accatgatgt ggtctgtcct
     4261 tttacagcca gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg gctggttgcc
     4321 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
     4381 ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga tacctcgcgg aaaacttggc
     4441 cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc
     4501 gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca
     4561 aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg
     4621 gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg
     4681 atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga
     4741 ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca
     4801 ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca
     4861 gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac
     4921 cctcccggtc gagtgagcga ggaagcacca gggaacagca cttatatatt ctgcttacac
     4981 acgatgcctg aaaaaacttc ccttggggtt atccacttat ccacggggat atttttataa
     5041 ttattttttt tatagttttt agatcttctt ttttagagcg ccttgtaggc ctttatccat
     5101 gctggttcta gagaaggtgt tgtgacaaat tgccctttca gtgtgacaaa tcaccctcaa
     5161 atgacagtcc tgtctgtgac aaattgccct taaccctgtg acaaattgcc ctcagaagaa
     5221 gctgtttttt cacaaagtta tccctgctta ttgactcttt tttatttagt gtgacaatct
     5281 aaaaacttgt cacacttcac atggatctgt catggcggaa acagcggtta tcaatcacaa
     5341 gaaacgtaaa aatagcccgc gaatcgtcca gtcaaacgac ctcactgagg cggcatatag
     5401 tctctcccgg gatcaaaaac gtatgctgta tctgttcgtt gaccagatca gaaaatctga
     5461 tggcacccta caggaacatg acggtatctg cgagatccat gttgctaaat atgctgaaat
     5521 attcggattg acctctgcgg aagccagtaa ggatatacgg caggcattga agagtttcgc
     5581 ggggaaggaa gtggtttttt atcgccctga agaggatgcc ggcgatgaaa aaggctatga
     5641 atcttttcct tggtttatca aacgtgcgca cagtccatcc agagggcttt acagtgtaca
     5701 tatcaaccca tatctcattc ccttctttat cgggttacag aaccggttta cgcagtttcg
     5761 gcttagtgaa acaaaagaaa tcaccaatcc gtatgccatg cgtttatacg aatccctgtg
     5821 tcagtatcgt aagccggatg gctcaggcat cgtctctctg aaaatcgact ggatcataga
     5881 gcgttaccag ctgcctcaaa gttaccagcg tatgcctgac ttccgccgcc gcttcctgca
     5941 ggtctgtgtt aatgagatca acagcagaac tccaatgcgc ctctcataca ttgagaaaaa
     6001 gaaaggccgc cagacgactc atatcgtatt ttccttccgc gatatcactt ccatgacgac
     6061 aggatagtct gagggttatc tgtcacagat ttgagggtgg ttcgtcacat ttgttctgac
     6121 ctactgaggg taatttgtca cagttttgct gtttccttca gcctgcatgg attttctcat
     6181 actttttgaa ctgtaatttt taaggaagcc aaatttgagg gcagtttgtc acagttgatt
     6241 tccttctctt tcccttcgtc atgtgacctg atatcggggg ttagttcgtc atcattgatg
     6301 agggttgatt atcacagttt attactctga attggctatc cgcgtgtgta cctctacctg
     6361 gagtttttcc cacggtggat atttcttctt gcgctgagcg taagagctat ctgacagaac
     6421 agttcttctt tgcttcctcg ccagttcgct cgctatgctc ggttacacgg ctgcggcgag
     6481 cgctagtgat aataagtgac tgaggtatgt gctcttctta tctccttttg tagtgttgct
     6541 cttattttaa acaactttgc ggttttttga tgactttgcg attttgttgt tgctttgcag
     6601 taaattgcaa gatttaataa aaaaacgcaa agcaatgatt aaaggatgtt cagaatgaaa
     6661 ctcatggaaa cacttaacca gtgcataaac gctggtcatg aaatgacgaa ggctatcgcc
     6721 attgcacagt ttaatgatga cagcccggaa gcgaggaaaa taacccggcg ctggagaata
     6781 ggtgaagcag cggatttagt tggggtttct tctcaggcta tcagagatgc cgagaaagca
     6841 gggcgactac cgcacccgga tatggaaatt cgaggacggg ttgagcaacg tgttggttat
     6901 acaattgaac aaattaatca tatgcgtgat gtgtttggta cgcgattgcg acgtgctgaa
     6961 gacgtatttc caccggtgat cggggttgct gcccataaag gtggcgttta caaaacctca
     7021 gtttctgttc atcttgctca ggatctggct ctgaaggggc tacgtgtttt gctcgtggaa
     7081 ggtaacgacc cccagggaac agcctcaatg tatcacggat gggtaccaga tcttcatatt
     7141 catgcagaag acactctcct gcctttctat cttggggaaa aggacgatgt cacttatgca
     7201 ataaagccca cttgctggcc ggggcttgac attattcctt cctgtctggc tctgcaccgt
     7261 attgaaactg agttaatggg caaatttgat gaaggtaaac tgcccaccga tccacacctg
     7321 atgctccgac tggccattga aactgttgct catgactatg atgtcatagt tattgacagc
     7381 gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg ctgctgatgt gctgattgtt
     7441 cccacgcctg ctgagttgtt tgactacacc tccgcactgc agtttttcga tatgcttcgt
     7501 gatctgctca agaacgttga tcttaaaggg ttcgagcctg atgtacgtat tttgcttacc
     7561 aaatacagca atagtaatgg ctctcagtcc ccgtggatgg aggagcaaat tcgggatgcc
     7621 tggggaagca tggttctaaa aaatgttgta cgtgaaacgg atgaagttgg taaaggtcag
     7681 atccggatga gaactgtttt tgaacaggcc attgatcaac gctcttcaac tggtgcctgg
     7741 agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa ttttcgatcg tctgattaaa
     7801 ccacgctggg agattagata atgaagcgtg cgcctgttat tccaaaacat acgctcaata
     7861 ctcaaccggt tgaagatact tcgttatcga caccagctgc cccgatggtg gattcgttaa
     7921 ttgcgcgcgt aggagtaatg gctcgcggta atgccattac tttgcctgta tgtggtcggg
     7981 atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt tgagaagacc tctcgggtat
     8041 ggtcaggtaa tgaacgtgac caggagctgc ttactgagga cgcactggat gatctcatcc
     8101 cttcttttct actgactggt caacagacac cggcgttcgg tcgaagagta tctggtgtca
     8161 tagaaattgc cgatgggagt cgccgtcgta aagctgctgc acttaccgaa agtgattatc
     8221 gtgttctggt tggcgagctg gatgatgagc agatggctgc attatccaga ttgggtaacg
     8281 attatcgccc aacaagtgct tatgaacgtg gtcagcgtta tgcaagccga ttgcagaatg
     8341 aatttgctgg aaatatttct gcgctggctg atgcggaaaa tatttcacgt aagattatta
     8401 cccgctgtat caacaccgcc aaattgccta aatcagttgt tgctcttttt tctcaccccg
     8461 gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc ctttacagat aaagaggaat
     8521 tacttaagca gcaggcatct aaccttcatg agcagaaaaa agctggggtg atatttgaag
     8581 ctgaagaagt tatcactctt ttaacttctg tgcttaaaac gtcatctgca tcaagaacta
     8641 gtttaagctc acgacatcag tttgctcctg gagcgacagt attgtataag ggcgataaaa
     8701 tggtgcttaa cctggacagg tctcgtgttc caactgagtg tatagagaaa attgaggcca
     8761 ttcttaagga acttgaaaag ccagcaccct gatgcgacca cgttttagtc tacgtttatc
     8821 tgtctttact taatgtcctt tgttacaggc cagaaagcat aactggcctg aatattctct
     8881 ctgggcccac tgttccactt gtatcgtcgg tctgataatc agactgggac cacggtccca
     8941 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg
     9001 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgataatca gactgggacc
     9061 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accatggtcc cactcgtatc
     9121 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
     9181 tctggaacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc
     9241 actcgtatcg tcggtctgat tattagtctg ggaccacgat cccactcgtg ttgtcggtct
     9301 gattatcggt ctgggaccac ggtcccactt gtattgtcga tcagactatc agcgtgagac
     9361 tacgattcca tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa cctgtagaac
     9421 ggagtaacct cggtgtgcgg ttgtatgcct gctgtggatt gctgctgtgt cctgcttatc
     9481 cacaacattt tgcgcacggt tatgtggaca aaatacctgg ttacccaggc cgtgccggca
     9541 cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg acgagctcgc gagctcggac
     9601 atgaggttgc cccgtattca gtgtcgctga tttgtattgt ctgaagttgt ttttacgtta
     9661 agttgatgca gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg
     9721 atggcctcca cgcacgttgt gatatgtaga tgataatcat tatcacttta cgggtccttt
     9781 ccggtgatcc gacaggttac ggggcggcga cctcgcgggt tttcgctatt tatgaaaatt
     9841 ttccggttta aggcgtttcc gttcttcttc gtcataactt aatgttttta tttaaaatac
     9901 cctctgaaaa gaaaggaaac gacaggtgct gaaagcgagc tttttggcct ctgtcgtttc
     9961 ctttctctgt ttttgtccgt ggaatgaaca atggaagtcc gagctcatcg ctaataactt
    10021 cgtatagcat acattatacg aagttatatt cgat
//
