LOCUS       sequence-119860-        8525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pJH110 variant Hygromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH122
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(20..25,26..43,44..50)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    58..74
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     63..85
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     82..98
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     82..98
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    117..150
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             complement(678..1703)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(678..1703)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2160..2837)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             2844..2855
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(2864..2898)
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     protein_bind    2905..2938
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(2973..2990)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2973..2989)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2982..3004)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3494..4288
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3548..3567)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4158..4177
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        4334..4779
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(4780..4866,4867..6201)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     oriT            6555..6664
                     /note="incP origin of transfer"
     promoter        complement(6899..6989)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(7183..7273)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      7633..8221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8122..8141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8375..8392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(8509..8525,1..5)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
       61 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgtgc
      121 cagggcgtgc ccttgggctc cccgggcgcg tgaaataagt ggctgcgcgc ataaagcggg
      181 tgatgggact aacgcctgac tagtaacata gatgacaccg cgcgcgataa tttatcctag
      241 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
      301 gtaaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
      361 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
      421 aaactttatt gccaaatgtt tgaacgatcg gggatccaga tcccggacaa tcgtgccctg
      481 gggaccaaca caatccagaa gggcctgaat cactgcgacc ggccctcccg cgacccagcc
      541 gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg
      601 catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg
      661 ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
      721 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
      781 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
      841 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
      901 cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag
      961 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     1021 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     1081 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     1141 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     1201 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     1261 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     1321 ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc
     1381 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     1441 gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     1501 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     1561 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     1621 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     1681 gcggtgagtt caggcttttt catatcttgc cccccgggat ctgttgtaag gagtctagag
     1741 cttggaggtc tgattttctc agtctccaga gatgtgttta tataggtagt aacctttttg
     1801 atatcagcca caagtgtgtg ggaatcttat cttcggattt caattaggaa ttaaccttat
     1861 tgaattctct tgaaaggaag tccgcaaagt ggttgtcggt tcctttaata tgttcaacat
     1921 caaacgaata gtggctaagc catgcttgcc atctgatgtt tcttccaagt tttgaatctc
     1981 ctttgtaatt aaggttaaca aaactcttga aatgagtatt atctgtccta atcagaaaat
     2041 gaacaggagt tagataaata ctgaatttct ttatagtatt tattaccgcc aatgtctctt
     2101 tgtcattgct gtggtaattc gcatgcctgc agcgatctac actagcacta tcagcgttat
     2161 taagcaccgg tggagtgacg accttcagca cgttcgtact gttcaacgat ggtgtagtct
     2221 tcgttgtggg aggtgatgtc cagtttgatg tcggttttgt aagcacccgg cagctgaacc
     2281 ggttttttag ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc gtctttcagt
     2341 ttcagacgca ttttgatttc acctttcaga gcaccgtctt ccgggtacat acgttcggtg
     2401 gaagcttccc aacccatggt ttttttctgc ataaccggac cgtcggacgg gaagttggta
     2461 ccacgcagtt taactttgta gatgaactca ccgtcttgca gggaggagtc ctgggtaacg
     2521 gtaacaacac caccgtcttc gaagttcata acacgttccc atttgaaacc ttccgggaag
     2581 gacagtttca ggtagtccgg gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac
     2641 tggaactgcg gggacaggat gtcccaagcg aacggcagcg gaccaccttt ggtaactttc
     2701 agtttagcgg tctgggtacc ttcgtacgga cgaccttcac cttcaccttc gatttcgaac
     2761 tcgtgaccgt taacggaacc ttccatacga actttgaaac gcatgaactc tttgataacg
     2821 tcttcggagg aagccatcta gtatttctcc tctttctcta gtagctagca caatacctag
     2881 gactgagcta gccgtcaagc atgccgcgcc cggggagccc aagggcacgc cctggcacct
     2941 ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     3001 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3061 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3121 ggcgaatggc gataagctag cttcacgctg ccgcaagcac tcagggcgca agggctgcta
     3181 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     3241 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     3301 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     3361 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     3421 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     3481 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3541 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3601 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3661 cctgaatgaa ctccaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3721 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3781 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3841 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3901 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3961 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4021 gcggatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4081 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4141 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4201 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4261 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgctag aggatcgatc
     4321 ctttttaacc catcacatat acctgccgtt cactattatt tagtgaaatg agatattatg
     4381 atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa
     4441 aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa
     4501 atccttttat gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga
     4561 gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc
     4621 aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta
     4681 ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc
     4741 agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca
     4801 aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc aactcaagcg
     4861 tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca
     4921 cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agtttctgaa
     4981 tttgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc
     5041 tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt
     5101 gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa
     5161 gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac
     5221 aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc
     5281 acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac
     5341 ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac
     5401 atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat
     5461 gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat
     5521 cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact
     5581 gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca
     5641 tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag
     5701 ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg
     5761 atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt
     5821 aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac
     5881 ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca
     5941 tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta
     6001 acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca
     6061 agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc gccgagcttc
     6121 ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga
     6181 caattaacag ttaacaaata aaaacgcaaa agaaaatgcc gatgggtacc gagcgaaatg
     6241 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6301 gaaaggttgg gcttcggaat cgttttccgg gacgccctcg cggacgtgct catagtccac
     6361 gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc
     6421 ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat
     6481 aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
     6541 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
     6601 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
     6661 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
     6721 tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca
     6781 gggttatgca gcggaaaagc gctgcttccc tgctgttttg tggaatatct accgactgga
     6841 aacaggcaaa tgcaggaaat tactgaactg aggggacagg cgagagacga tgccaaagag
     6901 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     6961 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     7021 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     7081 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     7141 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctcctg aaaatctcga
     7201 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     7261 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     7321 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     7381 gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
     7441 gtgctccagt ggcttctgtt tctatcaggg ctggatgatc ctccagcgcg gggatctcat
     7501 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     7561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8281 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8341 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8401 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8461 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8521 gctca
//
