LOCUS       sequence-119874-        4033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Device: FACS Standard Color Controls - High RFP (first unit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm:Gm_AF
FEATURES             Location/Qualifiers
     source          1..4033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..39
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             114..122
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             132..809
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      825..896
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      912..939
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        950..984
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             1059..1067
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1077..1790
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDYMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1107..1135)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1730..1750
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1809..1880
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1896..1923
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1941..1961
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1967..1984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2138..2726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2218..2237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2897..3688,3689..3757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3520..3539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3758..3862)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3930..3948
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3952..3995)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3998..4019
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagttgaca gctagctcag tcctaggtac tgtgctagct actgggccca agttcactta
       61 aaaaggagat caacaatgaa agcaattttc gtactgaaac atcttaatca tgctaaggag
      121 gttttctaat gatggcttcc tccgaggatg ttatcaaaga gttcatgcgt ttcaaagttc
      181 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
      241 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
      301 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
      361 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
      421 tgaacttcga agatggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
      481 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
      541 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggag gatggtgctc
      601 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
      661 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
      721 tcaaactgga catcacctcc cacaacgagg actacaccat cgttgaacag tacgaacgtg
      781 ctgaaggtcg tcactccacc ggtgcttaat aaaggtccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatagcttt tgacagctag
      961 ctcagtccta ggtactgtgc tagctactgg gcccaagttc acttaaaaag gagatcaaca
     1021 atgaaagcaa ttttcgtact gaaacatctt aatcatgcta aggaggtttt ctaatgatgc
     1081 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1141 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1201 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     1261 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
     1321 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1381 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1501 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1561 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1621 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1681 ccacacaatc tgccctttcg aaagatccca acgaaaagag agactacatg gtccttcttg
     1741 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataaaggt
     1801 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1861 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1921 gcgtttatac gctagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact
     1981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3901 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
     3961 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagaga
     4021 ctagtgggtc tca
//
