LOCUS       sequence-119896-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR88-Tango
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg actaacagct caagtacgag cactagttca acgactggag
      301 gaagtctgtt gctcttgtgc gaagaggagg aatcctgggc tggaagaagg ataccagtgt
      361 cactgctgta ctccggactg gctatcggcg gcacactggc gaatggcatg gtgatttatt
      421 tggtatcctc cttcagaaaa cttcagacaa cctctaacgc attcattgtt aacggatgcg
      481 ctgccgacct cagcgtgtgc gctctgtgga tgccccaaga agccgtcctg gggcttcttc
      541 cgaccggcag cgccgagcca cccgcggatt gggacggagc aggcggaagc taccgacttc
      601 tgcggggagg cctgctgggc ctgggactta ccgtgagcct gttgagtcat tgtttggtag
      661 cattgaacag gtatctcctt atcacacggg cccccgccac gtaccaggca ctgtaccagc
      721 ggcgccacac cgccggaatg ctggcacttt catgggcgct ggccctcggc ctggtcttgc
      781 tcttgccacc atgggcacca cggcccgggg cagctccacc gagggtgcac tatcctgccc
      841 tcctcgccgc tgctgctctg ctggctcaaa cagcactgct ccttcactgt tacctgggta
      901 tagtaagaag ggtcagggtc agcgtgaagc gcgtgtccgt gctgaatttt catttgctcc
      961 accagctgcc tggatgcgcg gctgctgccg ctgctttccc c
//
