LOCUS       sequence-119899-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: HCA2-Tango
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg aaccggcacc acctgcagga tcacttcctg gaaatagaca
      301 aaaagaactg ctgcgttttt agagacgact ttatcgtcaa agtactgccc ccagtgctcg
      361 gcctggagtt catcttcgga cttctgggga atggcctcgc cctctggatt ttttgcttcc
      421 accttaagtc ctggaagagt agccgaatct tcctgttcaa tctcgccgta gcggattttc
      481 tgctcatcat ttgcctgcct ttcctgatgg acaattatgt gcgccggtgg gattggaaat
      541 tcggtgatat tccttgtaga ttgatgctct tcatgctggc tatgaaccgg caaggaagca
      601 taatctttct taccgttgtg gccgtggaca gatacttccg agtggtgcat ccacatcatg
      661 ccctcaataa gatttccaat cgaacggccg ctataattag ctgtcttctg tggggaatca
      721 ccatcggcct caccgtccac ctcctcaaga agaagatgcc gatccagaac ggtggggcca
      781 acctgtgtag ttcctttagt atatgccata ctttccagtg gcatgaggct atgttcctgc
      841 tggagttctt cctgccactt ggcatcatct tgttctgctc cgctcggata atatggagcc
      901 tgagacagag acagatggat cggcatgcga aaataaagcg ggccattacg ttcatcatgg
      961 tcgtggctat tg
//
