LOCUS       sequence-119903-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CGG-R12-KIR RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-CGG-R12-KIR
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(74..88,89..94,95..111,112..118)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    94..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             191..208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attgttcatg accatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
       61 tcacctcgag aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga
      121 ttcaattgtg agcggataac aatttcacac agaattcatt aaagaggaga aattaactat
      181 gagaggatcg catcaccatc accatcacgg atccaacacg attaacatcg ctaagaacga
      241 cttctctgac atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga
      301 gcgtttagct cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg
      361 cttccgcaag atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc
      421 caagcctctc atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga
      481 ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa
      541 gccggaagcc gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga
      601 caatacaacc gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg
      661 cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact
      721 caacaagcgc gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat
      781 gctctctaag ggtctactcg gtggcgaggc gtggtcttcg tggcataagg
//
