LOCUS       sequence-119921-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..109
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(113..121,122..136)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..220
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     433..454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(560..581)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gccaccatgg gttctcatca
       61 tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta
      121 cgacgatgac gataaggatc tcgccaccat ggtcgactca tcacgtcgta agtggaataa
      181 gacaggtcac gcagtcagag ctataggtcg gctgagctca ctcgagaacg tctatatcac
      241 cgccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acgtggagga
      301 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      361 gctgctgccc gacaaccact acctgagcca tcagtccgtg ctttcgaaag accccaacga
      421 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      481 ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt
      541 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      601 cgtgaggggc gagggtgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      661 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca gctacggcgt
      721 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      781 gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacga
//
