LOCUS       sequence-119940-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  short photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: sspa-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..114
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     327..348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(454..475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgcca ccatggtcga ctcatcacgt
       61 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
      121 aacgtctata tcaccgccga caagcagaag aacggcatca aggcgaactt caagatccgc
      181 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      241 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgtgctttcg
      301 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      361 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
      421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      481 ggccacaagt tcagcgtgag gggcgagggt gagggcgatg ccaccaacgg caagctgacc
      541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      601 ttcagctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcctt caaggacgac
      721 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt g
//
