LOCUS       sequence-119948-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             114..809
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKATSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagc gaggagctga
      121 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca
      181 aggcaacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg
      301 gcagcaagac cttcatcgac cacacccagg gcatccccga cttcttcaag cagtccttcc
      361 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggcagcc tcatctacaa cgtcaagatc agaggggtgg
      481 acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg
      541 agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg
      601 tgggcgggag ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta
      661 agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg
      721 ccaacgacga gacctacgtc gagcagcacg aggtggcagt ggccagatac agcgacctcc
      781 ctagcaaact ggggcacaag cttaattaag cggccgcgac tctagatcat aatcagccat
      841 accacatt
//
