LOCUS       sequence-119949-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CTGA-R13-AKSRV RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-CTGA-R13-AKSRV
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..42
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(66..80,81..86,87..103,104..110)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             153..164
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             183..200
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaccatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg
       61 agaaatcata aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg
      121 tgagcggata acaatttcac acagaattca ttaaagagga gaaattaact atgagaggat
      181 cgcatcacca tcaccatcac ggatccaaca cgattaacat cgctaagaac gacttctctg
      241 acatcgaact ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag
      301 ctcgcgaaca gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca
      361 agatgtttga gcgtcaactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc
      421 tcatcactac cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga
      481 aagctaagcg cggcaagcgc ccgacagcct tccagttcct gcaagaaatc aagccggaag
      541 ccgtagcgta catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa
      601 ccgttcaggc tgtagcaagc gca
//
