LOCUS       sequence-119951-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. 
            PU6 driven sgRNA. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Norris AD, Kim HM, Colaiacovo MP, Calarco JA
  TITLE     Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of
            Dual-Marker Selection Cassettes.
  JOURNAL   Genetics. 2015 Oct;201(2):449-58. doi: 10.1534/genetics.115.180679. 
            Epub 2015 Jul 30.
  PUBMED    26232410
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.180679"; journalName: "Genetics"; date: 
            "2015-10"; volume: "201"; issue: "2"; pages: "449-58"
COMMENT     Sequence Label: pHKMC1: Empty sgRNA for Cloning
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(70..92)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     192..211
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     405..427
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     419..436
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     420..436
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        443..942
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        964..1039
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
       61 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
      181 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
      241 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
      301 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      361 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      421 taaaacgacg gccagtgaat tcctccaaga actcgtacaa aaatgctctg aagtaggtct
      481 cgagatcaat actgggaaga cgaaagtctt gcgaaaccga ttcgctgacc ccagtaaagt
      541 ctacttcggt agcccttccc ccaccaccca gctcgacgac gtcgacgagt acatctacct
      601 cggtcgtcaa atcaacgccc aaaacaactt gatgccggaa atccaccgaa gacgtcgagc
      661 agcctgggct gcattcaatg gaatcaagaa tgccaccgac tccatcaccg acaagaagat
      721 tcgtgcgaat ctgttcgact caattgtcct tccagcgctc acctacgttt cagaagcctg
      781 gacattcacc aaagctctat ccgaacgagt acgaatcaca catgcctccc ccacaaatta
      841 ttgatctaca tccccatttg gtatagtgtc ttacaggact ctcaatatgt cagctgcatg
      901 ataaatggtc cctatataat tgaattgcaa atctaaatgt ttgcggccgc ttaccggatc
      961 catgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     1021 agtggcaccg agtcggtgct ttttttcaat aatattctag atatcccttt ttg
//
