LOCUS       sequence-119952-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescently tagged Caveolin-3 truncation mutant for expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Luetterforst R, Stang E, Zorzi N, Carozzi A, Way M, Parton RG
  TITLE     Molecular characterization of caveolin association with the Golgi 
            complex: identification of a cis-Golgi targeting domain in the 
            caveolin molecule.
  JOURNAL   J Cell Biol. 1999 Jun 28;145(7):1443-59.
  PUBMED    10385524
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1999-06-28"; volume: "145(7)"; pages: "1443-59"
COMMENT     Sequence Label: EGFP-Caveolin3DGV
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..43,44..46,47..757)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(41..43,44..46,47..757)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(86..107)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(347..366)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     694..715
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga
       61 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      121 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      181 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      241 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      301 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      361 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      421 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      481 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      541 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      601 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      661 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      721 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
      781 agcttcgaat tcggacggtg tatggaaggt gagcttcacc acgttcaccg tctccaagta
      841 ctggtgctac cgcctgttgt ctacactgct gggtgttcca ctggccctgc tctggggatt
      901 cctgttcgcc tgtatctcct tctgccacat ctgggccgtg gtgccctgca ttaagagcta
      961 cctgatcgag atccagtgca tcagccacat ctactcactg tgtatccgca ccttctgcaa
     1021 cccgctcttc gctgcgttgg gccaggtctg cagcaacatt aaggtggtgc tgcgaaggga
     1081 aggc
//
