LOCUS       sequence-119960-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human FbxO11-Delta-Fbox in mammalian cells with FLAG-HA 
            tag at N-terminus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-FbxO11-Delta-Fbox-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg gttgcagaag
      181 aatcaggtcc tggtgcacaa aatagtccat accaacttcg tagaaaaact cttttgccga
      241 aaagaacagc gtgtcccaca aagaacagta tggagggcgc ctcaacttca actacagaaa
      301 actttggtca tcgtgcaaaa cgtgcaagag tgtctggaaa atcacaagat ctatcagcag
      361 cacctgctga acagtatatg gaagtatttg aatatactcg ccctatgatg catcctgaac
      421 ctggaaaatt ctaccagatt aatccagaag agtatgaaca tccaaatccc tggaaagaga
      481 gtttccagca gttgtataaa ggtgcacatg taaagccagg atttgctgaa catttctaca
      541 gtaaccctgc aagatataaa ggaagagaaa atatgttgta ttatgatact attgaagatg
      601 cccttggtgg ggtacaagag gctcattttg atggacttat ctttgttcat tctggaatat
      661 atactgatga atggatatat attgaatctc caatcaccat gattggtgca gcacctggga
      721 aagtggcaga caaagttata attgaaaaca ctagagattc aaccttcgtt tttatggaag
      781 gctctgaaga tgcttatgtt ggatatatga caataaggtt taaccctgat gacaaatctg
      841 cacaacacca caatgcacac cactgcttag agattacagt aa
//
