LOCUS       sequence-119963-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA 
            tag at N-terminus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-FbxO11-Jeff-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg gttgcagaag
      181 aatcaggtcc tggtgcacaa aatagtccat accaacttcg tagaaaaact cttttgccga
      241 aaagaacagc gtgtcccaca aagaacagta tggagggcgc ctcaacttca actacagaaa
      301 actttggtca tcgtgcaaaa cgtgcaagag tgtctggaaa atcacaagat ctatcagcag
      361 cacctgctga acagtatctt caggagaaac tgccagatga agtggttcta aaaatcttct
      421 cttacttgct ggaacaggat ctttgtagag cagcttgtgt atgtaaacgc ttcagtgaac
      481 ttgctaatga tccaattttg tggaaacgat tatatatgga agtatttgaa tatactcgcc
      541 ctatgatgca tcctgaacct ggaaaattct accagattaa tccagaagag tatgaacatc
      601 caaatccctg gaaagagagt ttccagcagt tgtataaagg tgcacatgta aagccaggat
      661 ttgctgaaca tttctacagt aaccctgcaa gatataaagg aagagaaaat atgttgtatt
      721 atgatactat tgaagatgcc cttggtgggg tacaagaggc tcattttgat ggacttatct
      781 ttgttcattc tggaatatat actgatgaat ggatatatat tgaatctcca atcaccatga
      841 ttggtgcagc acctgggaaa gtggcagaca aagttataat tgaaaacact agagattcaa
      901 ccttcgtttt t
//
