LOCUS       sequence-119965-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human FbxO10 in mammalian cells with FLAG-HA tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-FbxO10-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg gaggctggtg
      181 gcctcccctt ggagctgtgg cgcatgatct tagcctactt gcaccttccc gacctgggcc
      241 gctgcagcct ggtatgcagg gcctggtatg aactgatcct cagtctcgac agcacccgct
      301 ggcggcagct gtgtctgggt tgcaccgagt gccgccatcc caattggccc aaccagccag
      361 atgtggagcc tgagtcttgg agagaagcct tcaagcagca ttaccttgca tccaagacat
      421 ggaccaagaa tgccttggac ttggagtctt ccatctgctt ttctctattc cgccggagga
      481 gggaacgacg taccctgagt gttgggccag gccgtgagtt tgacagcctg ggcagtgcct
      541 tggccatggc cagcctgtat gaccgaattg tgctcttccc aggtgtgtac gaagagcaag
      601 gtgaaatcat cttgaaggtg cctgtggaga ttgtagggca ggggaagttg ggtgaagtgg
      661 ccctgctggc cagcattgat cagcactgct caaccacacg cctgtgcaac ctcgtcttca
      721 cgccagcctg gttctcaccc atcatgtata agacaacatc aggtcacgtc cagtttgaca
      781 actgcaactt tgagaacggg cacatccagg tccatggccc gggtacttgc caagtgaagt
      841 tctgtacctt caaaaacacc catatcttcc tgcacaacgt gcccctgtgt gtcctggaaa
      901 actgtgaatt tgtgggcagt gaaaa
//
