LOCUS       sequence-119974-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-mRFP-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(298..316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     566..585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     760..783
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg gcctcctccg
      181 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      241 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      301 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      361 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      421 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      481 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      541 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      601 cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc
      661 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
      721 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
      781 acgaggacta caccatc
//
