LOCUS       sequence-119977-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-mRFP-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..687)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(10..687)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(72..95)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(270..289)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     539..557
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(709..735)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(751..774)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(800..819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(801..819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcggatcct taggcgccgg tggagtggcg gccctcggcg cgctcgtact gttccacgat
       61 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg tcggtcttgt aggcgccggg
      121 cagctgcacg ggcttcttgg ccatgtaggt ggtcttgacc tcggcgtcgt agtggccgcc
      181 gtccttcagc ttcagcctca tcttgatctc gcccttcagg gcgccgtcct cggggtacat
      241 ccgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
      301 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
      361 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      421 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      481 ggagccgtac tggaactgag gggacaggat gtcccaggcg aagggcaggg ggccgccctt
      541 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
      601 gatctcgaac tcgtggccgt tcacggagcc ctccatgcgc accttgaagc gcatgaactc
      661 cttgatgacg tcctcggagg aggccattct agaacctcca cctccaccag cgtaatctgg
      721 aacatcgtat gggtaacctc caccgccccc tttatcgtca tcgtctttgt agtccatggt
      781 ggctagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca
      841 gtgggttctc tagttag
//
