LOCUS       sequence-119988-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 VPS29
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(683..707)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(708..731)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(774..795)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggtaccacg cgtacccaac acacggacac atgcacgaac acgcatgcag agagatcata
       61 catcatctat agagaagtac acgtaaaagt aagactcgtc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggtagatggc aaagttaaac gggtacgaaa ttctgctcgc tacttcctgt
      241 tctgcatccg agaggccagc ttccaccact acaccccaac cagcgggagg tgctttttgc
      301 gggaaacgag taggaaccgt ctggaaacgg aggaccgtgc ccctatcccc ggaacattcg
      361 aggtatagga ccaaaggcct tccctggctc gggcgtgcgt gtctcgtgac aggtcgggct
      421 gctagagggg cctttttacc ccttggatgg cctctggccc cttggggccg ggatacgaga
      481 cctaggccag ccgcacccag aggtggccgc tcccttcctt ccctagacct cttctgggcc
      541 tttcccaggc cagcgcctgg gccctgacgc cgaggcctcc gggatttccc aattcctcgc
      601 cctcggcctc cccacggctc ccaacaacgc agcaccccac ccgagagcca ggccttggtc
      661 gccgcaactg cagccctcac catgccaact tttttgtaca aacttgtcgg accctggaag
      721 agcacctcca gcttgtatag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      781 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      841 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      901 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      961 ttgatgccgt tcttctgctt g
//
