LOCUS       sequence-119989-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 VPS29
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..34
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(549..573)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      797..843
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(921..937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(921..937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    946..962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gagacttcag ctggaggtgc tcttccaggg tccgacaagt ttgtacaaaa aagttggcat
       61 ggtgagggct gcagttgcgg cgaccaaggc ctggctctcg ggtggggtgc tgcgttgttg
      121 ggagccgtgg ggaggccgag ggcgaggaat tgggaaatcc cggaggcctc ggcgtcaggg
      181 cccaggcgct ggcctgggaa aggcccagaa gaggtctagg gaaggaaggg agcggccacc
      241 tctgggtgcg gctggcctag gtctcgtatc ccggccccaa ggggccagag gccatccaag
      301 gggtaaaaag gcccctctag cagcccgacc tgtcacgaga cacgcacgcc cgagccaggg
      361 aaggcctttg gtcctatacc tcgaatgttc cggggatagg ggcacggtcc tccgtttcca
      421 gacggttcct actcgtttcc cgcaaaaagc acctcccgct ggttggggtg tagtggtgga
      481 agctggcctc tcggatgcag aacaggaagt agcgagcaga atttcgtacc cgtttaactt
      541 tgccatctac ccaactttct tgtacaaagt ggtgtaagcg gccgccctcc tcctcctttc
      601 ttgttccttt cacgtcgcct tctcggttgt agctggcaga cgacgagtct tacttttacg
      661 tgtacttctc tatagatgat gtatgatctc tctgcatgcg tgttcgtgca tgtgtccgtg
      721 tgttgggtac gcgtggtacc ctgcaggaag gaagctgagt tggctgctgc caccgctgag
      781 caataactag taattactag cataacccct tggggcctct aaacgggtct tgagggggtt
      841 ttttgctgaa aggaggacag ctgatgattg tcatgcttgc catctgtttt cttgcaaggt
      901 cagaggaatt cgtaatcatg gtcatagctg tttcctgtgt gaaaattgtt atccgctcac
      961 aattccacac aacatacgag ccggaagcat
//
