LOCUS       sequence-119991-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ISG15
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(688..712)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(713..736)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(779..800)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtagctccgc ccgccaggct ctgtgccgcc tccccgcagg cgcagattca
      241 tgaacacggt gctcaggggc ttgaggccgt actcccccag cgggagctgg tcctccaggg
      301 gcttcccctc gaaggtcagc cagaacaggt cgtcctgcac accctccagc ccgctcactt
      361 gctgcttcag gtgggccacg gtctgcgtca gccgcacctc gtaggtgctg ctgcggccct
      421 tgttattcct caccaggatg ttcagaggtt cgtcgcattt gtccaccacc agcaggaccg
      481 tgctgccggg gcccaggccc tggctggcaa gggggaccct gtcctgcagc gccacaccgc
      541 tcgggtggac agccagacgc tgctggaagg cgtgcacgcc gatcttctgg gtgatctgcg
      601 ccttcagctc tgacaccgac atggagctgc tcagggacac ctggaattcg ttgcccgcca
      661 gcatcttcac cgtcaggtcc cagcccatgc caactttttt gtacaaactt gtcggaccct
      721 ggaagagcac ctccagcttg tatagctcgt ccatgccgag agtgatcccg gcggcggtca
      781 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      841 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      901 gctggtagtg gtcggcgagc tgcacgctgc cg
//
