LOCUS       sequence-119992-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-BLMP-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg ggtcaaggaa
      181 gtggggatga cggtgttccg ccggcaccgt tctcttcggc tgctgcggca gctcactcac
      241 cacctcattc tcccctttct gtcggcgttt cttctgcttc atctgccacg tcatcatctt
      301 ccacaccgcc atcatccacg tcacctgccg gagtttcagc gtcaggtgcg agaaacgttg
      361 aaactgattg gaagcagtcg ggagatgaga gtcttgccga attatgtata tttcatgttc
      421 cagacaaatc tgttagtttg ccaaatccaa agcgagcaga atgcacactt cctatgaacc
      481 taattctcaa gtcctcctca aaaaatcgaa agaaatcgtc aatatggtca tctgatcata
      541 ttccgagagg agtccgattt ggcccgctcg ttggagaaat tcgtttggta gatgttgaca
      601 cagcacttgt atgtcctgct gaagcatcaa tggctggagg tggtccagct caagaagatg
      661 ttccatttga tgaagctccg gaagagtgga agatctattc tccatcaggt ggcagattga
      721 ataaaacgat ttgtgtgaaa gatgatgcga gatcaaattg gatgaaatac gtggcagctg
      781 ctgaagagga agattttcag aatcttgttg ctgcacaaat tggaaatgat atctactttt
      841 acacagttaa aaagattgaa gcaaatactg aattatcatt ttggttcagc agggactacg
      901 caaggaaatt aaattattca acaagaccat acgtaagagt tcgaaggcca gctactca
//
