LOCUS       sequence-120003-        8272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-DRE-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..8272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             908..931
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             947..973
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3866..3883)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3872..4096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4142..4570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4229..4248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4439..4460
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4579..4599)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4584..4913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4764..4899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4826..4845
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4980..5774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5034..5053)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5644..5663
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5948..6069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5985..6004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6039..6058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6118..6134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6118..6134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6131..6153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6142..6158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6166..6172,6173..6190,6191..6196))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6211..6232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6349..6366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6520..7105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6600..6619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7276..8067,8068..8136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7899..7918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8137..8241)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggac tacaaagacg atgacgataa agggggcggt ggaggttacc catacgatgt
      961 tccagattac gctggtggag gtggaggttc tagactcgag cggccgctta tgtcgtcctc
     1021 ttcgtcacca ttcttccatc ctccacctca acaagtgatg gatgatacga cacagcaatc
     1081 gcccagttac tcacaaaact cgaattctcc atcacaacat acgttcgagt caatggaacc
     1141 tgctgctgga ggatcaacgt cgatgagata ttcaccatcg ggaagctccg aagcagacaa
     1201 tccgacactc ggattgtttt ctgcacaagc ctcttatcca tactccctcc gaaaacgtcg
     1261 gccttgtttg acaaaggatg aggatttgtg cttcccgatt acagctgcaa cagctgatga
     1321 gacacctcaa cccacgatga aaaagttcaa gctggaatgt ctttcacctg gcgaagctcg
     1381 agatttctcg attgaagacg agagcggcac aaacctggaa gccaaaatgg agacaacgga
     1441 atgtgaacca gaagaagagg aggcggtgga agaagaagat caacaagatc atatcaatag
     1501 actccccgaa gaacttcttc tcaaagtgtt ttcatttctt cctgataaat cgctgcttgc
     1561 atgtagttca gtctcatacc gattcaatca aatttcgaat agtcatgaag tgtggaagga
     1621 gctgtattgt aatttatatg actatcgaat tccacttttc cacccatctc atgcaaaatt
     1681 cgagttccgg gaacagagcc gttggcggga tggtaatcca tggaaagagt cccatcgaca
     1741 gcttcatcat ggagttcatg tgatgaaaga gccacgggtc aatcttcgat ctgtcaacta
     1801 tcgttgtttc gatcaaattg agaaggctca atcgttcttg gaggaggatg agtatagaga
     1861 gaaacttatt ttcttgcaca ctggtgttca tgagccaatc gacacaattc tcatcaacac
     1921 agatgtgcag attatcggag caagtgactc acgagatatc acatcttcgg ttgttctcga
     1981 aggaagtaaa aataccgcat tgacattcac tgatggaagt gcaaacgcgt atttcggatt
     2041 catcacagtc cggtttcggg ctgatccagt gtgcaggcag cagccacaga ttgctcagca
     2101 ggctcaacaa atgaatcatt tctattcgat tttagtgact gataaggatg caatgccata
     2161 tattgaaaga tgtgatatca cttcgaaagt tggaaatggt gcagctgtat gtgtgaagaa
     2221 aagtgcggct cctaaattca aatattgcac agttcttgat tgtgaaaatg ttggaatcta
     2281 cattactgac aacgccaccg gacactatga gcactgtgaa attgctcgaa atactttggc
     2341 tggtgtctgg gtgaaaaatc atgcgaatcc ctacttccga aagtgtacaa ttcattctgg
     2401 aaaggatgtg ggagttttca catttgaaca tggtcagggt tactttgaaa agtgcaatat
     2461 ccattcaaat cggatatctg gcatcgaggt gaaaaattcg gcaaatccgg ttgtgattcg
     2521 atgcgaagtg catcatggtt acactggagg tatctatgtt catgagagag gacgtggcca
     2581 gtttatggag aatcggatat atgcgaacgc gtatgcaggt atctggatca cttcgcattc
     2641 tgatccgact atccgaaaga atgagatttt cactggacaa cagggtggag tctacatctt
     2701 tggtgaagga cgaggactca tcgagcagaa taatatttat ggaaacgcgt tggctggaat
     2761 tcagatccgc tcacaatccg atccaatcgt aagattgaat aagattcacg atggattgca
     2821 cggaggaatt tatgttcacg agaaaggaag aggattgatt gaagagaatg aagtttatgg
     2881 aaatacgttg gcaggaatct gggtaacaac tggttcaagt ccaattctgc gcaaaaatag
     2941 aattcattct ggaaaacagg tcggagtata cttctacgat caaggtcacg gacttcttga
     3001 agaaaatgat attttcaatc atttgtattc cggagtgcaa attcgaacgg gatcaaaccc
     3061 aaaaatcaca aggaataaga tttggggagg tcagaatgga ggtgtactcg tgtacaatgg
     3121 aggaaaggga tgcttggaag acaatgaaat cttcgataat gcaatggcgg gtgtctggat
     3181 caaaacagac agtgagccaa ctcttcgacg aaacaagatc tacgacggaa gagatggcgg
     3241 agtttgtatt ttcaatcgtg gtaaaggtct tctcgaggac aatgaaatct tccgaaatgc
     3301 gcaagctggt gttctcattt caaccgaatc gaatccaact ctacgaagaa atcgagtatt
     3361 tgatggaaaa tcggcgggaa tcgagatcac gaatggagcg acagctacac tagaagaaaa
     3421 tcaattgttc agaaataaat atggaggact ttgcgtagca accggtgtca ctccagtgca
     3481 acgtggaaat cgtatctatg acaaccatga cacaatttct agagctatca agactggcct
     3541 gtgtctcttc aaagtgagca gcaacaacag ttttccgatg cacaatttct atagatgtac
     3601 cacgtgtaac acgacggaac gtaatgcgat ctgcaccaac tgcatcagaa catgccatcg
     3661 tggtcattct gtagaacttg tgaggttcga tagattcttc tgcgactgtg gtgctggaac
     3721 tctcgaacgg cactgtcacc tgcaaaatgt gccacgtgac aacgacactg tctatgactc
     3781 agcgacgccg atctccactg agactggcac cgaaatttaa ggatccgagc tcggtaccaa
     3841 gcttaagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3901 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3961 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4021 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4081 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4141 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4561 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4681 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4741 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4801 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4861 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4921 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4981 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5041 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5101 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5161 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5221 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5281 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5341 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5401 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5461 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5521 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5581 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5641 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5701 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5761 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5821 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5881 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5941 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6061 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6121 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6181 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6241 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6301 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6361 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6421 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6481 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6541 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6601 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6661 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6721 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6781 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6841 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6901 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6961 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7021 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
     7081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
