LOCUS       sequence-120005-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-DRE-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagactc gagcggccgc
      181 ttatgtcgtc ctcttcgtca ccattcttcc atcctccacc tcaacaagtg atggatgata
      241 cgacacagca atcgcccagt tactcacaaa actcgaattc tccatcacaa catacgttcg
      301 agtcaatgga acctgctgct ggaggatcaa cgtcgatgag atattcacca tcgggaagct
      361 ccgaagcaga caatccgaca ctcggattgt tttctgcaca agcctcttat ccatactccc
      421 tccgaaaacg tcggccttgt ttgacaaagg atgaggattt gtgcttcccg attacagctg
      481 caacagctga tgagacacct caacccacga tgaaaaagtt caagctggaa tgtctttcac
      541 ctggcgaagc tcgagatttc tcgattgaag acgagagcgg cacaaacctg gaagccaaaa
      601 tggagacaac ggaatgtgaa ccagaagaag aggaggcggt ggaagaagaa gatcaacaag
      661 atcatatcaa tagactcccc gaagaacttc ttctcaaagt gttttcattt cttcctgata
      721 aatcgctgct tgcatgtagt tcagtctcat accgattcaa tcaaatttcg aatagtcatg
      781 aagtgtggaa ggagctgtat tgtaatttat atgactatcg aattccactt ttccacccat
      841 ctcatgcaaa attcgagttc cgggaacaga gccgttggcg ggatggtaat ccatggaaag
      901 agtcccatcg acagcttcat catggagttc atgtgatgaa agagccacgg g
//
