LOCUS       sequence-120014-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-BLMP-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg agaggatcgc atcaccatca ccatcacggg ccctctagaa
      121 tgggtcaagg aagtggggat gacggtgttc cgccggcacc gttctcttcg gctgctgcgg
      181 cagctcactc accacctcat tctccccttt ctgtcggcgt ttcttctgct tcatctgcca
      241 cgtcatcatc ttccacaccg ccatcatcca cgtcacctgc cggagtttca gcgtcaggtg
      301 cgagaaacgt tgaaactgat tggaagcagt cgggagatga gagtcttgcc gaattatgta
      361 tatttcatgt tccagacaaa tctgttagtt tgccaaatcc aaagcgagca gaatgcacac
      421 ttcctatgaa cctaattctc aagtcctcct caaaaaatcg aaagaaatcg tcaatatggt
      481 catctgatca tattccgaga ggagtccgat ttggcccgct cgttggagaa attcgtttgg
      541 tagatgttga cacagcactt gtatgtcctg ctgaagcatc aatggctgga ggtggtccag
      601 ctcaagaaga tgttccattt gatgaagctc cggaagagtg gaagatctat tctccatcag
      661 gtggcagatt gaataaaacg atttgtgtga aagatgatgc gagatcaaat tggatgaaat
      721 acgtggcagc tgctgaagag gaagattttc agaatcttgt tgctgcacaa attggaaatg
      781 atatctactt ttacacagtt aaaaagattg aagcaaatac tgaattatca ttttggttca
      841 gcagggacta cgcaaggaaa tt
//
