LOCUS       sequence-120016-        7885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-BLMP-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..7885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             917..934
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3479..3496)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3485..3709
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3755..4183
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3842..3861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4052..4073
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4192..4212)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4197..4526
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4377..4512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4439..4458
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4593..5387
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4647..4666)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5257..5276
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5561..5682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5598..5617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5652..5671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5731..5747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5731..5747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5744..5766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5755..5771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5779..5785,5786..5803,5804..5809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5824..5845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5962..5979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6133..6718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6213..6232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6889..7680,7681..7749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7512..7531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7750..7854)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgaga ggatcgcatc accatcacca tcacgggccc tctagaatgg gtcaaggaag
      961 tggggatgac ggtgttccgc cggcaccgtt ctcttcggct gctgcggcag ctcactcacc
     1021 acctcattct cccctttctg tcggcgtttc ttctgcttca tctgccacgt catcatcttc
     1081 cacaccgcca tcatccacgt cacctgccgg agtttcagcg tcaggtgcga gaaacgttga
     1141 aactgattgg aagcagtcgg gagatgagag tcttgccgaa ttatgtatat ttcatgttcc
     1201 agacaaatct gttagtttgc caaatccaaa gcgagcagaa tgcacacttc ctatgaacct
     1261 aattctcaag tcctcctcaa aaaatcgaaa gaaatcgtca atatggtcat ctgatcatat
     1321 tccgagagga gtccgatttg gcccgctcgt tggagaaatt cgtttggtag atgttgacac
     1381 agcacttgta tgtcctgctg aagcatcaat ggctggaggt ggtccagctc aagaagatgt
     1441 tccatttgat gaagctccgg aagagtggaa gatctattct ccatcaggtg gcagattgaa
     1501 taaaacgatt tgtgtgaaag atgatgcgag atcaaattgg atgaaatacg tggcagctgc
     1561 tgaagaggaa gattttcaga atcttgttgc tgcacaaatt ggaaatgata tctactttta
     1621 cacagttaaa aagattgaag caaatactga attatcattt tggttcagca gggactacgc
     1681 aaggaaatta aattattcaa caagaccata cgtaagagtt cgaaggccag ctactcaact
     1741 gattccatct gcaccaccag cttctgcatc aactgcaatt gcgagtttag cagaaacaat
     1801 tgtggcaatt gattattcag ttaaaaaact cattgaatca cccattgata cactctctac
     1861 tgatgcttct tctgcaagtg acgaagaaat gattgatgtt gaagagcagg agagctgtac
     1921 acggcctgtt gcagaagtca cacggcctaa tgttatccag aatccagttg tgaggcctgt
     1981 tgcaaccaaa gtcaataatt tcccgggaat acccgttcga cttggcaatt tttatgcaag
     2041 tccgttggtg gattttaaag agttcatgag gaaatccctt caattaaaac tagttgatac
     2101 ctcaatgttt gtgtcccctg ttgctcaaac aacagccgca ataactgcca ctggtggaag
     2161 atccggccag ccgattgatg tgcagccagt acttgcagca actgcaggag ctcattttgg
     2221 aaactatgca gcaatttatg ggagtcaaga tttccagcat gagctatcaa aacctctgta
     2281 cacatctgcg tctccagcat tcggaggtgg tggtggtatg ggtggaggat ttggaatggg
     2341 tggaagtgcc catacttctt catttcatca acttccattt gttaatcatt cgtcttcatc
     2401 acacaatgat tcatcattta atggagttcc aaattacgtg caacaacagg aaaatggaaa
     2461 aacgagatac gcttgcaaag actgcaacaa aacattcgga cagctttcga atcttaaagt
     2521 tcatgtcaga acgcacaccg gcgaacgtcc attcaaatgt gaaatttgca caaaagagtt
     2581 cacacagctt gctcatcttc agaagcatca tcttgttcat accggagaac gtcctcatcg
     2641 ctgtgatatt tgtgataagc gattctcgtc gacttctaat ttaaaaactc atcttcgact
     2701 tcacaatggt cagaagccgt atacgtgtga tgtttgtgat gccaagttca ctcaatacgt
     2761 tcatctaaga cttcataaac gtctgcatgc aaacgaaaga ccgtatagtt gtggaacatg
     2821 tggaaagaaa tatatttcac cgagtggatt gagaactcat tggaaaacaa ctacatgtaa
     2881 agaagaggat atgaaagact cgatgaggga tgatttaatg gatatcaaag gcgaaatcga
     2941 tgaaggatca atgtcagggt cgggttatgg aaatctggga atctttgaaa atacactgaa
     3001 ctccgaactc aagcggccac ttatgccaat cgaaaccatt tatagtaaat acaatcttcc
     3061 caacgcctcc ctcctcggtc aaggtccaag tggaatgcaa gaacaacagg ctccaccacc
     3121 aacatctcaa cagcagcaac acatgatgta tggaaacaca atgggacata tgggacaagg
     3181 atcccatctt caggggccac caccacctcc acaacatttc caaatggatc atagtggaat
     3241 gcagaatgga ggaggtattc cacatcaaca tcaacttatt caaggaggtc catcatctgg
     3301 atctggtcaa cagcaacatc cacaacataa tgggattcat aggcttcctg atttgaaaaa
     3361 tcccctactt ccaagcctcg gattgccgca ttatccataa gaattctgca gaattccacc
     3421 acactggact agtggatccg agctcggtac caagcttaag tttaaacccg ctgatcagcc
     3481 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3541 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3601 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3661 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3721 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3841 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3901 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3961 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4021 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4081 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4141 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4201 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4261 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4321 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4381 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4441 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4501 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4561 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4621 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4681 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4741 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4801 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4861 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4921 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4981 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5041 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5101 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5161 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5221 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5281 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5341 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5401 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5461 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5521 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5581 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5641 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5701 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5761 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5821 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5881 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5941 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6001 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6061 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6541 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6661 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7861 tccccgaaaa gtgccacctg acgtc
//
