LOCUS       sequence-120018-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ATF3
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(736..760)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(761..784)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(827..848)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtagctctgc aatgttcctt cttttatctg ttggataaag aggtttctct
      241 catcttctgg agtcctccca ttctgagccc ggacaataca cgtgggccga tgaaggttga
      301 gcatgtatat caaatgctgc ttctcgttct tgagctcctc aatctgagcc ttcagttcag
      361 cattcacact ttccagcttc tccgactctt tctgcaggca ctccgtcttc tccttcttct
      421 tgtttcggca ctttgcagct gcaatcttat ttctttctcg tcgcctcttt ttcctttcat
      481 cttcttcagg ggctacctcg gcttttgtga tggacacccc gaggggtctg tcgctgacag
      541 tgactgattc cagcgcagag gacatccggt ggcagaggtg cttgttctgg atggcaaacc
      601 tcagctcttc cttgacaaag ggcgtcaggt tagcaaaatc ctcaaacacc agtgacccag
      661 gaggggacag gcaggggacg atggcagaag cactcacttc cgaggcagag acctggcctg
      721 ggtgttgaag aatcatgcca acttttttgt acaaacttgt cggaccctgg aagagcacct
      781 ccagcttgta tagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      841 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      901 agtggttgtc gggcagcagc acgggggc
//
