LOCUS       sequence-120019-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-mRFP-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..704)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(27..704)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(89..112)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(287..306)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     556..574
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(717..734)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(769..788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(770..788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataatagctg gtacgagctc ggatccttag gcgccggtgg agtggcggcc ctcggcgcgc
       61 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttgatgtcg
      121 gtcttgtagg cgccgggcag ctgcacgggc ttcttggcca tgtaggtggt cttgacctcg
      181 gcgtcgtagt ggccgccgtc cttcagcttc agcctcatct tgatctcgcc cttcagggcg
      241 ccgtcctcgg ggtacatccg ctcggtggag gcctcccagc ccatggtctt cttctgcatt
      301 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      361 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      421 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      481 tgcttcacgt aggccttgga gccgtactgg aactgagggg acaggatgtc ccaggcgaag
      541 ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      601 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgcgcacc
      661 ttgaagcgca tgaactcctt gatgacgtcc tcggaggagg ccattctaga gggcccgtga
      721 tggtgatggt gatgcgatcc tctcatggtg gctagccagc ttgggtctcc ctatagtgag
      781 tcgtattaat ttcnataanc cagtaagcag tgggttctct agttanccaa naaagctctg
      841 cttatatana cctcccancg tanaacncct ancgnccatt ttgcntcaat ggggcggaat
      901 tgttacnaaa nttttngaaa anncccgttg aattttggtg ncnaaaaaaa actcccnatt
      961 gacntnaang gggnnggaaa ncttgaaaaa tccccntgaa ntnaaaacnn ntanncancn
     1021 cccanttnan ttnactgnca aaaaccnnan tnnnna
//
