LOCUS       sequence-120020-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-DRE-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg agaggatcgc atcaccatca ccatcacggg ccctctagac
      121 tcgagcggcc gcttatgtcg tcctcttcgt caccattctt ccatcctcca cctcaacaag
      181 tgatggatga tacgacacag caatcgccca gttactcaca aaactcgaat tctccatcac
      241 aacatacgtt cgagtcaatg gaacctgctg ctggaggatc aacgtcgatg agatattcac
      301 catcgggaag ctccgaagca gacaatccga cactcggatt gttttctgca caagcctctt
      361 atccatactc cctccgaaaa cgtcggcctt gtttgacaaa ggatgaggat ttgtgcttcc
      421 cgattacagc tgcaacagct gatgagacac ctcaacccac gatgaaaaag ttcaagctgg
      481 aatgtctttc acctggcgaa gctcgagatt tctcgattga agacgagagc ggcacaaacc
      541 tggaagccaa aatggagaca acggaatgtg aaccagaaga agaggaggcg gtggaagaag
      601 aagatcaaca agatcatatc aatagactcc ccgaagaact tcttctcaaa gtgttttcat
      661 ttcttcctga taaatcgctg cttgcatgta gttcagtctc ataccgattc aatcaaattt
      721 cgaatagtca tgaagtgtgg aaggagctgt attgtaattt atatgactat cgaattccac
      781 ttttccaccc atctcatgca aaattcgagt tccgggaaca gagccgttgg cgggatggta
      841 atccatggaa agagtcccat cgacagcttc atcatggagt tcatgtgatg aaagagccac
      901 gggtcaatct tcgatctgtc aactatcgtt gtttcgatca a
//
