LOCUS       sequence-120022-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-BLIMP-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg agaggatcgc atcaccatca ccatcacggg ccctctagaa
      121 tgttggatat ttgcttggaa aaacgtgtgg gtacgacctt ggctgccccc aagtgtaact
      181 ccagcactgt gaggtttcag ggattggcag aggggaccaa ggggaccatg aaaatggaca
      241 tggaggatgc ggatatgact ctgtggacag aggctgagtt tgaagagaag tgtacataca
      301 ttgtgaacga ccacccctgg gattctggtg ctgatggcgg tacttcggtt caggcggagg
      361 catccttacc aaggaatctg cttttcaagt atgccaccaa cagtgaagag gttattggag
      421 tgatgagtaa agaatacata ccaaagggca cacgttttgg acccctaata ggtgaaatct
      481 acaccaatga cacagttcct aagaacgcca acaggaaata tttttggagg atctattcca
      541 gaggggagct tcaccacttc attgacggct ttaatgaaga gaaaagcaac tggatgcgct
      601 atgtgaatcc agcacactct ccccgggagc aaaacctggc tgcgtgtcag aacgggatga
      661 acatctactt ctacaccatt aagcccatcc ctgccaacca ggaacttctt gtgtggtatt
      721 gtcgggagtt tgcagaaagg cttcattacc cttatcccgg agagctgaca atgatgaatc
      781 tcacacaaac acagagcagt ctaaagcaac cgagcactga gaaaaa
//
