LOCUS       sequence-120025-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enables eukaryotic expression of N-terminally RGS-6xHis–tagged 
            fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(220..237)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    226..450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      496..854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(583..602)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     793..814
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg agaggatcgc atcaccatca ccatcacggg ccctctagac
      121 tcgagcggcc gccactgtgc tggatatctg cagaattcca ccacactgga ctagtggatc
      181 cgagctcggt accaagctta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt
      241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
      481 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      541 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      601 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      661 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      721 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      781 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      841 attcttttga tttataagg
//
