LOCUS       sequence-120026-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enables eukaryotic expression of N-terminally RGS-6xHis–tagged 
            fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(86..415)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      100..235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(154..173)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     400..420
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(429..857)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(539..560)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     751..770
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatccgaaaa
       61 tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc
      121 actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa
      181 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
      241 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
      301 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
      361 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
      421 acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      481 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
      541 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
      601 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
      661 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      721 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      781 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      841 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      901 agccatagag cc
//
