LOCUS       sequence-120038-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RAP2A
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(786..809)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(852..873)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagctgg gttactcgag ctattgtatg ttacatgcag aacagcatgg gtcatctttg
      241 tcaggctgag cagcatagtt catctgcctc acaatttctg caaagagttc gtccaccatt
      301 gttttactct tagcggaagt ttccataaag gggcagcccc actcttcagc aagggctctg
      361 ccttcgctgg acgatacttc tctctcactt tccaggtcca ctttgttccc aaccaagatg
      421 actggcactt tctcataccg cttcacgcgg atgatctggt cccgcatggg cttgatgtcc
      481 tggaagctct gctggttgac gaggctgtag acgaggatga agccctggcc gttcttgatg
      541 tacaggtccc gcatggacgc gaactgctcg gtgcccgccg tgtccaggat ctccagcacc
      601 gacggcgacg aatccacctc gatctccttg cggtagaagt cctcgatggt ggggtcgtat
      661 ttctcgatga aggtgccggt cacgaactgc acggtcaggg cggatttgcc taccccgccc
      721 gagcccagca ccaccacttt gtactcgcgc atgaattccg agcctgcttt tttgtacaaa
      781 cttgtcggac cctggaagag cacctccagc ttgtatagct cgtccatgcc gagagtgatc
      841 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      901 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      961 atgggggtgt tctgctg
//
