LOCUS       sequence-120043-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 BTF3
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(679..703)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(704..727)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(770..791)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcaggttgcc tcattcttgg aagcctcatc aaaattctcc acaagatctg
      241 gaacttcatc atcatcatcc tctccagtag caagtggtgc ttttccatcc acagattgtt
      301 tgggcagagc ttcggccagt ctccttaaac tagtcagact atccgcacca agctggttta
      361 agatgctggg tagcatttct gtcagctgct ttgtctcagc atggcctgta atggtgaaag
      421 tgttcgctgc cagagatgcc tgaactttag ggttgttaaa gtggatcact gttccttggt
      481 ttgtaaacat attcacctct tcaataccag agatattgtt tacccctaac ttctttaagg
      541 agaactgaag ttttttgtca tctgctgtgg ctgttctatg aaccaccttc ttctttctgc
      601 gagcagttcc tttcccacca atgcgcactt gtgcctgcag tttggcgagt ttttcctggt
      661 tcatgattgt ttctttcatg ccaacttttt tgtacaaact tgtcggaccc tggaagagca
      721 cctccagctt gtatagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      781 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
      841 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      901 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtgg
//
