LOCUS       sequence-120047-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL6
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(829..853)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(854..877)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(920..941)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtacatctgc cgaagagccc tcaggctgga ctgcaggaac tccttaaagc
      241 tgcgcagaat gagatgagtt gtcatgtcct gcagccactg gttctgtgcc tgcagcttcg
      301 tcagcaggct ggcatttgtg gttgggtcag gggtggttat tgcatctaga ttctttgcct
      361 ttttctgcag gaactggatc aggacttttg tactcatctg cacagctctg gcttgttcct
      421 cactactctc aaatctgttc tggaggtact ctaggtatac ctcaaactcc aaaagaccag
      481 tgatgatttt caccaggcaa gtctcctcat tgaatccaga ttggaagcat ccatcttttt
      541 cagccatctt tggaaggttc aggttgtttt ctgccagtgc ctctttgctg ctttcacaca
      601 tgttactctt gttacatgtc tcctttctca gggctgagat gccgtcgagg atgtaccgaa
      661 tttgtttgtc aattcgttct gaagaggtga gtggctgtct gtgtggggcg gctacatctt
      721 tggaatcttc tcctgggggt actggggcag ggaaggcagc aggcaacacc aggagcagcc
      781 ccagggagaa ggcaactgga ccgaaggcgc ttgtggagaa ggagttcatg ccaacttttt
      841 tgtacaaact tgtcggaccc tggaagagca cctccagctt gtatagctcg tccatgccga
      901 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
//
