LOCUS       sequence-120060-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: NPBW1-Tango
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
      121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgcg ccaccatgaa
      181 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      241 tgacgccagc atcgatatgg acaacgccag tttcagcgag ccctggcccg ccaacgcaag
      301 cggaccagac cctgctctta gctgctcaaa tgcttccacc ctggcccctc tgccggctcc
      361 cctcgccgtt gccgtccctg tcgtgtacgc agtgatctgt gcagtcggtc tggccgggaa
      421 cagtgccgtg ctttacgtgc tgctcagagc cccgaggatg aagactgtca ctaacctctt
      481 tattctgaac ctcgcgatcg ccgacgaact cttcacattg gttctcccca tcaacatcgc
      541 agatttcctc ctgcgacaat ggcctttcgg cgagctcatg tgcaaactta tcgtcgccat
      601 agatcagtac aacactttta gtagtctgta ctttctgacc gtgatgtcag ccgaccgcta
      661 cctcgtggta ctggctactg ccgagtccag gagagttgcg ggtaggacgt atagcgctgc
      721 ccgagccgta tctttggctg tgtggggaat tgtgacgctg gtggtgttgc catttgccgt
      781 ttttgcccgc ctcgacgacg agcagggccg ccggcaatgc gtgctggtat ttccacagcc
      841 agaagcgttt tggtggcgag cgtcccggtt gtatacgctg gtgctgggtt tcgctattcc
      901 cgtgtctacc atttgcgtac tttacaccac tctgctctgt cgcctgcacg caatgagact
      961 ggatagccac gccaaggcac tggaacgggc caaaaaac
//
