LOCUS       sequence-120088-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of S. enterica MTAN in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Hemeon I, Gutierrez JA, Ho MC, Schramm VL
  TITLE     Characterizing DNA methyltransferases with an ultrasensitive 
            luciferase-linked continuous assay.
  JOURNAL   Anal Chem. 2011 Jun 15;83(12):4996-5004. doi: 10.1021/ac200816m. 
            Epub 2011 May 16.
  PUBMED    21545095
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac200816m";
            journalName: "Anal Chem"; date: "2011-06-15- 15"; volume: "83"; 
            issue: "12"; pages: "4996-5004"
COMMENT     Sequence Label: Salmonella MTAN
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..39
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(750..767)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(774..791)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat catcaccact ttgtacaaga aagctgggtg gatcctatta accgtgcgcc
       61 agtttttgaa ccagcgtttc caccatcagg gtagactgtt tagctgcaac cgccaggaat
      121 tcatcgaaag acaggtggga ttgctggtct gcaacatcag agatggcgcg aaccacaaca
      181 aacggcacgt tgaagttgtg gcaaacgtgg gcgattgcgg tggcctccat ttctaccgcg
      241 accgcgtccg ggaagttgtg acggatttta gccagaccaa cggagccgtt gatgaaggca
      301 tcgccggaca cgatcagacc acgaacagcg ttcaggttca gttcacggat gcaagactct
      361 gccgctgcga tcagtttatc atcagcctta aagcctgccg gacagccagg cagctgaccg
      421 tattcgtaac cgaatgcggt cacgtctgca tcgtgataac gggtttcgtc gctgacgaca
      481 atatcaccaa ccttcagagt gcttgccaga ccacccgcgg agccggtgtt gatgatcaca
      541 tccggtttgc agtgttccag cagcagggtt gcacccagcg cggctgccac tttaccaatg
      601 ccagatttca gcagagctac ttcagtaccg ttcagctggc cggtataaat ctcgcaacca
      661 cccagagtaa tcgtctggcg attgtcgatc ttatcacgca gcagagtcac ttcctcctcc
      721 atcgcgccga tgataccgat tttcatatgg gagccgcgtg gaaccaggcc gccgtgatga
      781 tgatggtggt gagaaccgcc catggttaat tcctcctgtt ctttctagaa gcctgcttt
//
