LOCUS       sequence-120101-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..821)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKATSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttaat taagcttgtg ccccagtttg ctagggaggt cgctgtatct ggccactgcc
      181 acctcgtgct gctcgacgta ggtctcgtcg ttggcctcct tgattctttc cagtctgtag
      241 tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga tctatatgtg
      301 gtcttggcgt ttgcgatcag atggctcccg cccacgagct tcagggccat gtcgtttctg
      361 ccttccaggc cgccgtcagc ggggtacagc gtctcggtga aggcctccca gccgagtgtt
      421 ttcttctgca tcacagggcc gttggatgtg aagtccaccc ctctgatctt gacgttgtag
      481 atgaggctgc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg
      541 tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa gaagtcgggg
      601 atgccctggg tgtggtcgat gaaggtcttg ctgccgtaga ggaagctagt agccaggatg
      661 tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc
      721 tcgtagggct tgccttcgcc ctcggatgtt gccttgaagt gatggttgtc cacggtgccc
      781 tccatgtaca gcttcatgtg catgttctcc ttaatcagct cctcgctcat ggtggcgacc
      841 ggtggatcc
//
