LOCUS       sequence-120107-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..78
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(82..90,91..105)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..189
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     402..423
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(529..550)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gaaggagata gatctatggg ttctcatcat catcatcatc atggtatggc tagcatgact
       61 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg
      121 gtcgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg
      181 ctgagctcac tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
      241 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
      301 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
      361 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      421 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
      481 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      541 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
      601 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      661 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      721 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga g
//
