LOCUS       sequence-120109-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: NPBW2-Tango
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccacctgc ttactgggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gcgccaccat
      181 gaagacgatc atcgccctga gctacatctt ctgcctggta ttcgccgact acaaggacga
      241 tgatgacgcc agcatcgata tgcaggcagc gggccaccca gaacccctcg atagccgagg
      301 gtccttttcc ctgcccacga tgggcgccaa tgtatctcag gacaacggga ctggccataa
      361 cgctactttc tccgaaccac tcccatttct gtatgtcctc ctgccagccg tttacagcgg
      421 gatctgcgca gtgggattga cgggtaacac agcagtgatc ctcgtcatcc tgcgagcacc
      481 caaaatgaag acggtgacca atgtgttcat ccttaatttg gctgtggccg acggactgtt
      541 tactctggtc ctgcccgtga acatagctga acacttgttg cagtactggc cgtttgggga
      601 actgttgtgt aaactggtgc tcgcagtcga ccattataat atattctctt ccatttactt
      661 tcttgctgtg atgagcgttg accgctatct ggtcgtactt gcgaccgtaa gatctcggca
      721 catgccctgg cgcacgtata ggggcgcaaa ggtggcctct ctgtgcgtct ggctcggggt
      781 gaccgtgctg gtgcttccct tcttcagttt tgccggtgtg tactccaacg aacttcaagt
      841 gccctcctgt ggcctgtcat tcccatggcc agaacaagtg tggttcaaag caagcagagt
      901 ctacacgttg gtactgggat ttgtcctccc agtgtgcacc atctgcgtgc tgtacacaga
      961 tctgctgcga aggctcagag ctgtta
//
