LOCUS       sequence-120111-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  short photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: sspa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..118
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     331..352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(458..479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gccaccatgg tcgactcatc
       61 acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact
      121 cgagaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcga acttcaagat
      181 ccgccacaac gtggaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      241 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgtgct
      301 ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      361 cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag
      421 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      481 aaacggccac aagttcagcg tgaggggcga gggtgagggc gatgccacca acggcaagct
      541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      601 caccttcagc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct ccttcaagga
      721 cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      781 catcgagctg aagggcatcg acttcaagga ggacggcaac atcct
//
