LOCUS       sequence-120120-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-20"; volume: "9(1):4902. doi"; pages: " 
            10.1038/s41598-019-41277-z"
COMMENT     Sequence Label: pF CAG luc hygro
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(131..149)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag gatccgccgc
       61 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga
      121 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      181 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
      241 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      301 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      361 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
      421 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      481 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
      541 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
      601 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc
      661 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
      721 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
      781 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
      841 atttcgagtc gtcttaatgt atagatt
//
