LOCUS       sequence-120121-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..84
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(88..96,97..111)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..195
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     408..429
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(535..556)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctttaagaag gagatagatc tatgggttct catcatcatc atcatcatgg tatggctagc
       61 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatctcgcc
      121 accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt cagagctata
      181 ggtcggctga gctcactcga gaacgtctat atcaccgccg acaagcagaa gaacggcatc
      241 aaggcgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
      301 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      361 agccatcagt ccgtgctttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
      421 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
      481 ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      541 gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg tgagggcgat
      601 gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      661 tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt gcttcagccg ctaccccgac
      721 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      781 accatctcct tcaaggacga cggcacctac aagacccgcg ccgagg
//
