LOCUS       sequence-120130-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: P2RY4-Tango
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gcgagcactg aatcttcact gctgagatcc ctgggactct
      301 cccccgggcc aggatctagc gaggtagaac tggactgttg gttcgacgag gactttaagt
      361 tcatcctgct tcccgttagc tacgccgtgg tgtttgtcct tggccttggg ctcaatgctc
      421 ccacactctg gcttttcata tttcgcctgc gaccctggga cgcgacagcc acctacatgt
      481 ttcatctggc cctgagcgac accttgtacg tcctcagtct gcctaccctg atctactatt
      541 atgccgcaca caaccactgg ccgttcggaa cagaaatctg caagtttgtg cgatttctgt
      601 tctattggaa cttgtattgt agtgttttgt ttcttacatg catttcagtg caccgctacc
      661 tgggcatttg tcatcccctg agagctctga ggtgggggag acctcggctc gctgggctcc
      721 tgtgcctggc tgtctggctg gtcgtggctg gatgcctggt acccaatctc ttcttcgtga
      781 cgacttctaa taagggcaca accgttctgt gtcatgacac aacccggcca gaagaattcg
      841 accactacgt ccatttttca agtgccgtga tgggcctgct gtttggggtg ccatgcctcg
      901 tgacactggt ctgttatggg ctgatggcta
//
