LOCUS       sequence-120131-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. 
            Cannot modify orientation of cat gene; promoter too weak in E. coli,
            probably transcribed via repB promoter in bicistronic manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA3b61
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    64..120
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(145..167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      182..268
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     811..830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggatctat caacaggagt ccaagactag tcgccagggt tttcccagtc acgacgcggc
       61 cgcaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
      121 gcgcggccgc ggcctaggcg gcctcctgtg tgaaattgtt atccgcttta attaaaggca
      181 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      241 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggc gcgcccccgt
      301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      841 cgggtttcgc cacctctgac ttgagcgtcg attt
//
